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Diane Bolton, Ph.D.

Chief, Animal Models and Pathogenesis

Dr. Diane Bolton received her Ph.D. from the NIH-Johns Hopkins University cooperative graduate program in 2006. She completed her postdoctoral fellowship on mucosal vaccine immunogenicity and gene expression analysis of virally infected cells with Dr. Mario Roederer at NIH’s Vaccine Research Center in Bethesda, Md.

Research

Dr. Bolton’s interests focus on immune responses and viral dynamics during HIV/SIV vaccination and infection. Specifically, her team aims to explore the complex immune responses to vaccine regimens, such as that used in RV144, to elucidate cellular and humoral correlates of protection. Using the rhesus macaque model of HIV infection allows investigation of a broad range of responses in multiple tissues, including mucosal sites critical for HIV/SIV transmission. In addition to studying the immune response to the virus, we examine viral mechanisms underlying transmission, disease pathogenesis, and chronic infection. HIV/SIV preferentially infects CD4 T cells, but the specific subsets of these or other hematopoietic cells that harbor virus in vivo remain unknown, particularly at the time of infection. Such cell populations represent important targets for both preventative vaccines and therapeutic curative interventions. By characterizing active and latent viral infection within individual cells during acute and chronic disease we hope to lay the foundation for discoveries that limit HIV infection and disease progression. Advances in single-cell flow cytometric and gene expression technologies enable MHRP researchers to address these questions at the unprecedented single-cell level.

Selected Publications

    1.    Priming T-cell responses with recombinant measles vaccine vector in a heterologous prime-boost setting in non-human primates. Bolton DL, Santra S, Swett-Tapia C, Custers J, Song K, Balachandran H, Mach L, Naim H, Kozlowski PA, Lifton M, Goudsmit J, Letvin N, Roederer M, Radošević K. Vaccine. 2012 Sep 7;30(41):5991-8.
    2.    Comparison of systemic and mucosal vaccination: impact on intravenous and rectal SIV challenge. Bolton DL, Song K, Wilson RL, Kozlowski PA, Tomaras GD, Keele BF, Lovingood RV, Rao S, Roederer M.  Mucosal Immunol. 2012 Jan;5(1):41-52.
    3.    Genetic immunization in the lung induces potent local and systemic immune responses. Song K, Bolton DL, Wei CJ, Wilson RL, Camp JV, Bao S, Mattapallil JJ, Herzenberg LA, Herzenberg LA, Andrews CA, Sadoff JC, Goudsmit J, Pau MG, Seder RA, Kozlowski PA, Nabel GJ, Roederer M, Rao SS. Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22213-8. doi: 10.1073/pnas.1015536108. Epub 2010 Dec 6. Erratum in: Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2629. Wei, Chih-Jen [added].
    4.    Trafficking, persistence, and activation state of adoptively transferred allogeneic and autologous Simian Immunodeficiency Virus-specific CD8(+) T cell clones during acute and chronic infection of rhesus macaques. Bolton DL, Minang JT, Trivett MT, Song K, Tuscher JJ, Li Y, Piatak M Jr, O'Connor D, Lifson JD, Roederer M, Ohlen C. J Immunol. 2010 Jan 1;184(1):303-14.
    5.    Flow cytometry and the future of vaccine development. Bolton DL, Roederer M. Expert Rev Vaccines. 2009 Jun;8(6):779-89.